By Dawson, MW; Smythe, LD; Scott, JG; Sutherland, CJ
An immunofluorescent microscopic method for specifically detecting and
counting airborne Thermoactinomyces sacchari spores has been developed. The
spores were indirectly labelled with (PITC) fluorescein isothiocyanate. The method
has been successfully field tested during stored bagasse reclamation, when the
downwind drift of airborne spores from the truck loading point was monitored. High
airborne spore levels were detected close to the loading point, although it was
apparent that local topography and weather conditions, particularly wind strength
and direction, had significant influence over the extent and direction of drift. In
addition, an ELISA blood test has been developed as an aid to diagnosis of suspected
bagassosis. Medical practitioners in cane growing areas will have access to
the ELISA, along with information on other diagnostic criteria and case management.
The development of the ELISA, used in conjunction with other tests such as
lung function, will enable large scale surveillance to be undertaken.