By BARRY J. CROFT, JANET GREEN, DON PARSONS, ALLAN ROYAL
BSES established laboratories to screen samples for ratoon stunting disease (RSD) with
an enzyme-linked immunosorbent assay (ELISA) in Tully in 1993 and Mackay in 1994.
The laboratories have processed more than 380 000 samples during the 10 years of
operation. The benefits to the sugar industry have been a greater confidence in the
disease-free status of plant sources and quality control programs for approved seed
plots. The laboratories were responsible for preventing widespread distribution of RSD-infected plant sources from seed plots on at least three occasions. The two districts that
have made most use of the laboratories are Mackay and Harwood. The Mackay district
has had a long history of successful control of RSD. The RSD laboratories have enabled
this region to maintain RSD below 2% of commercial fields throughout the period. This
position was maintained even during the orange rust epidemic when growers were
forced to use old ratoons for plant sources. Harwood had a severe RSD problem in the
early 1990s. Through the extensive use of the RSD laboratories combined with
increased use of approved seed and education of growers and planting contractors of the need for hygiene, Harwood has been able to reduce RSD from 40% of plant sources
infected to around 10%. The RSD laboratories have recently expanded to conduct
testing of preserved samples from Papua New Guinea, Indonesia, Malaysia and China.
The ELISA test for RSD is cost effective and enables intensive sampling that greatly
increases the chances of detecting the disease.